【摘要】 用选择性培养基从杉木幼苗根围分离细菌,发现根面的数量最多,比非根际土多达百倍;其中根面分离细菌中能离体拈抗立枯丝菌的分离物高达70％,而非根际土只只12.5％。利用紫外光诱变几个产生黄光的Pseudomonas分离物,能稳定地抗利福平,用以测定对杉木幼苗根面的附着,显示AP03~-菌株随时间延长(5—50分钟)附着的细胞增加,并依赖于初始菌的密度,在每毫升含10~3—10~7个细胞范围内,紧密附着于根面的细菌数呈线性关系。在根的提取物中发现具有凝聚AP03~-等菌株的凝聚因(但不凝聚大肠杆菌),推断是一种收白质。更多还原

【Abstract】 Isolates of fluorescent Pseudomonas were isolated from the seedling rhizoplane and rhizophere of China fir (Cunn-inghamia landeolata) With the fluorescent pseudomonad selec-tive media (Acc). The number of colonies from the rhizoplane was larger, and about 100 times more than that from the non-rhizosphere soil. the percentage of isolates, isolated from rhizoplane and in vitro antagonistic against Rhizoctonia solani on KB, reached 70%. the chosen strains, induced tolerant to rifampin by UV, Were used for binding assay. It wad found that the cells of AP03~+ adhesive to the root surface of China fir incrdased with time (from 5 to 60 minutes), and depended upon the starting concentration (form 10~3 to 10~7 cfu/ml) with a linear rdlation, the agglutinative factor in the root extracts was found out to agglutinate AP03~+ (not to E. coli), and was deduced as a protein.更多还原