【摘要】 以番茄叶片的染色体DNA为模板,用PCR技术扩增ACC合成酶基因的5′侧翼区,得到了2.0kb的特异性扩增片段,并将该片段克隆到了pBluescript KS M13—的EcoRV位点上。重组子的酶切图谱和插入片段两端的序列分析结构与Rottmann等报导的基本一致。该侧翼区的获得为进一步研究该基因的表达调控规律打下了基础。更多还原

【Abstract】 From total genomic DNA of tomato leaves, 2.0kb specific fragment was obtained by PCR amplification of ACC synthase gene 5′-flanking region and cloned into pBluescript KS M13-digested with EcoRV. The restriction maps of the recombinant and the sequence of both ends of the insert were essentially identical to the results reported by Rootmann. The availability of this flanking region makes it possible to study the expression mechanism of ACC synthase gene.更多还原