【摘要】 本文应用透射电镜观察了东方杯叶吸虫卵黄腺和卵巢的超微结构,并与体外培养成虫进行比较。根据形态特征和内含物的存在情况,将卵黄细胞和卵母细胞的发育均分为不同时期,详细描述了各期的形态特征,探讨了卵黄球和皮质颗粒等内含物的生理功能。体外培养成虫成熟卵黄细胞中有散在的卵黄物质,成熟卵母细胞中线粒体囊泡化,这些可作为体外培养的评价指标。更多还原

【Abstract】 By means of TEM, the vitelline follicles of Cyathocotyle orientalis are shown to be composed of cells in three stages of development. Immature vitelline cells are undifferentiated and possess a small amount of cytoplasm filled predominantly with free ribosomes. Developing vitelline cells are larger, containing Golgi bodies, extensive rough endoplasmic reticulum and free ribosomes. With continued protein synthesis, the cytoplasm becomes filled with large amounts of vitelline globules. Further maturation results in the disintegration of rough endoplasmic reticulum, Golgi bodies and mitochondrion. Mature vitelline cells contain free ribosomes, glycogen, lipid droplets, ribosomal complex and large clusters of vitelline globules at the periphery of the cell. The vitelline cell, therefore, develops and functions first as a shell protein secretory cell and subsequently as a food store for the developing embryo. Vitelline materials scatter in mature vitelline cells of ovigerous adults cultured in vitro and this may correspond to production of abnormal eggs in vitro.The ovary consists of a central mass of mature oocytes, which is surrounded by smaller im- mature oocytes. Mature oocytes are characterized by large clusters of mitochondrion and a sub-plasmalemmal layer of cortical granules. Vacuolation occurs in mitochondria of mature oocytes of the adults cultured in vitro.更多还原