【摘要】 本实验制备组成分别为DOPE/Chol/OA（4:4:3）的酸敏脂质体及DOPC/Chol/OA（4:4:3）脂质体,用于包裹质粒pSV₂-neo、pUC18-ras、pSV-neo-ras及大分子线状DNA,包裹率可达50％.被脂质体包裹的DNA不被DNase降解,提示DNA分子被脂质体包裹在微球体内部.电泳检测表明,被包裹的DNA分子没有断裂.所得到的脂质体较稳定,4℃放置5～6月,脂质体内仍保留部分DNA分子.制备酸敏脂质体时,pH为8.0时脂质体较稳定,pH<6.5～7时则不稳定.更多还原

【Abstract】 Liposomes entrapping plasmids pSV2-neo DNA, pUC18-ras DNA, pSV2-neo-ras DNA and linear DNA were prepared. The liposomes were composed of DOPC/Chol/OA (4 : 4 : 3) and pH-sensitive DOPE/Chol/OA (4:4:3), respectively. The efficiency of DNA entrapment was about 50%. Gel electrophoresis analysis showed: Liposome-entrapped DNA was not digested by DNase; The entrapped DNA molecules were intact and stable for at least 5-6 months at 4癈. During preparation of pH-sensitive liposome, the pH must be kept at 8.0.更多还原