【摘要】 本工作拟建立一种 HLA 基因组 DNA 定型方法,采用聚合酶链反应技术对所要分析的 HLA—Ⅱ DR4基因进行体外扩增,并配合等位基因特异性寡核苷酸探针对扩增产物进行分析,以确定个体是否带有该型等位基因.我们用该方法对上海地区52例正常人及32例类风湿关节炎病人 DR4基因进行了分析,结果显示正常人 DR4基因携带者为17.3%,而类风湿关节炎病人高达43.8%(RR=3.7,P<0.01),DR4阳性的病人多为重症活动者(X~2=11.7,P<0.005).此外,我们将该方法与血清学定型方法进行了比较.更多还原

【Abstract】 In this article,we describe a polymerase chain reaction（PCR）method for HLA DNA typing.Specftic primers were synthesixed and used to amplify the second exon of HLA-Ⅱ DR4 gene.Also the PCR products were hybridized ith ³²P—labeled DR4 sequence specific oligonucleotide probe.The results of DNA typing by PCR were compared with those typed by serological method.Fifty two normal individuals and thirty two rheumatoid arthritis（RA）patients were analysed by this method.The percentase of DR4 carriers in normal individuals is 17.3%,while in RA patients is 43.8%（RR=3.7,P<0.01）.Most of the DR4 positive patients are active and severe（X²=11.7,P<0.005）.The result confirms that DR4 gene is positively associated with RA.更多还原