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应用等速电泳法测定转移因子中ATP、c-AMP的含量

Isovelocity Phoresis Measuring ATP,cAMP of Transfer Factor

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【作者】 李竹; 朱光奇; 陈荣善;

【Author】 Li Zhu~1 Zhu Guangqi~2 Chen Rongshan (~1 Institute of Identification of Medicine and Biological Products of China; ~2 Department of Epidemiology,~3 Department of Microbiology,Hubei Medical College,Wuhan 430071,China)

【机构】 中国药品生物制品鉴定所; 湖北医学院流行病学教研室; 湖北医学院微生物学教研室;

【摘要】 应用等速电泳(IP)法检测了以人的脾脏、扁桃腺、白细胞为原料生产的四批转移因子(TF)中的 ATP 和 c—AMP 的含量,经多次复测,重复性好,变异系数均小于5%,适用于常规分析。

【Abstract】 ATP and cAMP of 4 batches of transfer factor (TF) purified from human being’s spleen,tonsil and WBC were measured by isovelocity phoresis (IP) method.After many repetitions,the results were similar.All the variation coefficients were <5%.We suggest that IP method could be as an analysis routine for measuring ATP and cAMP of TF.

【关键词】 转移因子/分离与提纯; 电泳;
【Key words】 transfer factor/IP; electrophoresis;
  • 【文献出处】 湖北医学院学报 , 编辑部邮箱 ,1992年03期
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