【摘要】 本文报道了维生素B₁（B₁）和维生素B₆（B₆）的光化学动力学（波长选择和时间选择）荧光分析法联合测定的原理和方法。该法的建立是基于B₁的光化学荧光衍生化反应和B₆的光分解反应。B₁和B₆的浓度分别在0～1.4μg/ml和0～2.0μg/ml呈良好的线性关系,相关系数皆为0.999。检出限分别为0.60ng/ml B₁和1.0ng/ml B₆（时间选择）或2.0ng/ml B₆（波长选择）。相对标准偏差分别为0.99％（n=8）和2.3％（n=8）。该法用于混合物的测定效果良好。更多还原

【Abstract】 A new method for simultaneous determinatien of vitamin B₁ and B₆ by photochemical kinetie （time-election and wavelength-election）fluorimetry is presented. It is based on the photochemical reaction of vitavnin B₁ and B₆ in NaOH medium with an increase in the fluorescence intensity of B₁ and a decrease in the fluorescence intensity of B₆. The prineiple of method is discussed. Linear eaiibraticn eurves are obtained over the range of 0～1.4 and 0～2.0μg/ml for vitamin B₁ and B₆, respectivety （r=0.999 for both）. The limits of determination were 0.60 ng/ml Ba₁ and 1.0ng/ml B₆（time-eloetibn）or 2.0 ng/ml B₆ （wavelength-election）. The relative standard deviations of determination for B₁ and B₆ were 0.99%（n=8） and 2.3% （n=8）, respectively. This method has been applied to the determination of mixture of vitamin B₁ and B₆ with satisfactory results更多还原