【摘要】 本文从卵巢上皮癌腹水免疫复合物中,解离出患者的自身抗原(OC₄₁)免疫小鼠,取其脾细胞用于融合,经ELISA、PAP及流式细胞计等方法筛选和鉴定,获得2株杂交瘤细胞系,分别命名为COC₄₁D和COC₄₁E,2种细胞诱生小鼠腹水,ELISA测定抗体滴度达2×10⁵和1×10⁵。免疫组化分析表明,COC₄₁D和COC₄₁E与卵巢上皮癌具有较高的特异性,阳性率分特为70.2％和52.5％。本研究表明,应用腹水免疫复合物解离抗原有可能获得特异性较高的单克隆抗体。更多还原

【Abstract】 We immunized BALB/c mice with antigens (OC₄₁) dissociated from immune complexes （ICs） ofepithelial ovarian cancer ascites. The spleen cells were used for fusion with Sp 2/0-Ag 14 murinemyeloma call line. By cloning and screening, two hybridoma cell lines were obtained from 10 fusions.named as COC₄₁ D and COC₄₁ E respectively. The titers of the 2 MAbs in the ascites of BALB/cmice were 2×10⁵ and 1×10⁵. Immunohistological analysis showed that COC₄₁ D and COC₄₁ E werepositively stained in paraffin embeded tissue sections of ovarian carcinoma as 70.2% and 52.8%tespectively, however, no cross-reactivity with varous kinds of benign and normal human tissuesections was tested. The use of antigens dissociated from autologous ICs as immunogens for preparinghigh specific anti-tumor MAbs may provide a valuable means in the stud of tumor antigens.更多还原