【摘要】 献血者血清标本共82份,使用不同的抗-HCV试剂盒,用两种方法（ELISA和PHA）进行对比检测。结果用混合抗原试剂盒A₁测得的抗-HCV阳性率为56.09％,用A₂为71.95％,A₃为47.56％,A_4-1为63.41％,B为59.75％。用单一抗原试剂盒A_4-2、A_4-3检出的阳性率分别为41.46％和62.19％。其中8份血清经过四基因重组免疫印迹法（RIBA）进行确证,A₁、A₂、A₃、A_4-1、A_4-3全部符合。RIBA阳性的76号血清A_4-2阴性,RIBA阴性的82号血清B为阳性。提示国产试剂虽可检出大部分抗-HCV阳性献血者,但在试剂的抗原匹配、组装、临界值确定等方面尚有许多问题有待解决。更多还原

【Abstract】 82 serum samples from the blood donors were comparatively tested by using different reagent kits and two methods (enzyme-linked immunosorbent assay, ELISA anl passive hemagglutination inhibition assay, PHA). The results are as follows: the positive rates of A1, A2 , A3 , A4-1 and B (containing mixed antigens) are 56.09%, 71.95%, 47.56%, 63.41% and 59.75% respectively; and the positive rates of A4-2 and A4-3 (containing single antigens) are 41.46% and 62.19% respectively. The results of testing 8 of all the serum samples by four-gene-recombinent immunoblot assay (RIBA) confirmed that the results of A1, A2, A3, A4-1 and A4-3 were consistent with them. The 76th sample with positive result when tested by RIBA had a negative result when tested by RIBA, while the 82th sample with negative result when tested by RIBA had posi tive one when tested by B. It is suggested that reagent kits made in China left much to be desired in antigen-matching, compactness and cut-off value determining, though they could screen out most of the donors with positive anti-HCV results.更多还原