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火焰原子吸收法测定红细胞膜结合钙

FLAME ATOMIC ABSORPTION SPECTROMETRY DETERMINA-TION OF BINDING CALCIUM IN HUMAN RED ERYTHROCYTE MEMBRANE

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【作者】 杨根元徐德选何亚楠陆德澄苏子杰凌玲

【Author】 Yang Genyuan, Xu Dexuan and He Yanan Department of Instrumental Analysis, Zhenjiang Medical College, 212001 Zhenjiang Lu Decheng, Su Zijie and Ling Ling Affiliated Hospital, Zhenjiang Medical College

【机构】 镇江医学院仪器分析教研室镇江医学院附属医院心血管科镇江医学院附属医院心血管科 212001 镇江212001 镇江212001 镇江

【摘要】 <正> 本文用火焰原子吸收法测定红细胞膜结合钙含量。测定健康者35人,膜结合钙平均值为4.68±0.45μg/mg膜蛋白;原发性高血压患者34人,膜结合钙平均值为2.00±0.73μg/mg膜蛋白。由测定结果表明,原发性高血压组胞膜结合钙含量明显低于对照组,差别高度显著(P<0.01)。该方法的回收率为98.23%—104.37%。相对标准偏差小于3.18%。红细胞膜钙泵的基本功能是将细胞膜内的Ca2+主动转运至细胞外,以维持细胞内低钙,使细胞内外电解质平衡处于正常生理状态。红细胞内的钙大部分结合在膜上。膜

【Abstract】 The present study was performed to determine the amount of binding calcium in human red erythroeyte membrane by using flame AAS. In thirty-five healthy subjects, the average of binding calcium in red erythrocyte membrane was 4.68-0.45μg/mg membrane proteins; In thirty-four subjects with primary hypertension, the average of binding calcium in red erythrocyte membrane was 2.00-0.73μg/mg membrane proteins. The result revealed that in subjects with primary hypertension, binding calcium in red erythrocyte membrane was significantly lower than that of the healthy controls (P<0.01). The rate of recovery of this method varied from 98.23% to 104.37% and the RSD was less than 3.18%.

  • 【文献出处】 光谱学与光谱分析 ,Spectroscopy and Spectral Analysis , 编辑部邮箱 ,1991年05期
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