【摘要】 介绍了一种先进的氚离子束标记技术,专门用于蛋白质、肤和非挥发性有机化合物的氚标记。在该技术中,用经加速受激发的氚离子束去轰击固体样品,发生氢-氚交换反应。已用此法制备了氚化大豆胰蛋白酶抑制剂、核糖核酸酶A、弹性蛋白和茯苓聚糖等11种化合物。氚化粗产物经透析、离子交换色层和凝胶过滤等方法纯化后,蛋白质和聚糖的比活性均可达到37GBq/mmol以上,具有天然的生物活性而没有放射性副产物。氚化蛋白质的氨基酸分析表明:在蛋白质中,His.、Tyr.和Phe.残基具有较高的比活性而Val.、Ile.和Ser.残基的比活性较低。更多还原

【Abstract】 An advanced technique, the ion beam tritium labelling method (IBTL) , used for labelling proteins, peptides and other nonvolatile organic compounds is introduced. In this method the excited tritium ion beam is accelerated and then bombs a solid sample target in which tritium exchanging for hydrogen is taken place. The IBTL has been used for preparation of tritiated soybean trypsin inhibitor, ribonuclease A, elastin and pachyman etc. After purifing by dialysis, ion exchange chromatography and gel filtration, the tritiated proteins and polysaccharide were obtained with the specific acitvity over 37 GBq/mmol, the function of tritiated decomposition products was not found. The product was shown to have native biological activity. Amino acid analysis of tritiated protein showed that the relative specific radioactivities were higher for His., Tyr. and Phe. but lower for Val. , He. and Ser.更多还原