【摘要】 用RHDV人工感染易感兔,每隔4小时剖杀一组,整个病程大多为24小时,且病变典型.血凝试验查得,主要脏器病毒出现时间顺序是肝(攻毒后12小时)、脾、心、肾(16小时)、肺(20小时);病程后期,病毒含量从高到低依次为肝、脾、肺、肾、心。免疫荧光间接法检查感染兔各脏器,发现病毒经肌注兔体后4小时,即可定位于肝细胞,并在肝细胞核内复制增殖;兔感染病毒后16小时,在肝、脾、肾小球中可检出有免疫复合物沉着或抗体附着。电镜检查,肝细胞核内发现有病毒粒子,直径为25～30nm,细胞结构破坏严重。作者认为,RHDV 是一种 DNA 病毒;免疫复合物引起的免疫损伤及肝细胞结构、功能被破坏是兔发生病毒性出血症的重要原因。更多还原

【Abstract】 Most rabbits died in 24 hours after being infected by RHDV,and thesymptoms were clear.In the test,a group of rabbits were killed every 4hours from 0 to 24 hours.HA test directed,the order of the appearance ofRHDV in the main tissues was liver(12 hours after infection ),spleen,heart,kidney(16 hours),lung(20 hours);the order of the RHDV contentin the main tissues before death was liver,spleen,lung,kidney,heart.Bythe inderect immunofluorescence technique,it was found that RHDV could bedetected in the liver-cells four hours after the muscle injection with RHDV,and it reproduced in the liver-cell’s nuclei,and there were immune complexesor antibodies deposits in or adhered to the liver-cells,spleen-cells,andglomeruli of the kidney 16 hours after the rabbit being infected.The electr-omicroscope technique showed,the virus particles were roughly spherical,about 25-30 nm in diameter,without envelope,and the livercells were destr-oyed seriously.The auther points out,RHDV is a kind of DNA virus;theimmune damage caused by the immune complexes and the destruction of liver-cells’ structure and function were the major causes of viral hemorrhagic dis-ease in rabbits.更多还原