【摘要】 考察了测定水生腐殖酸分子量分布及其平均分子量的串联凝胶色谱法。结果表明,使用Sepbadex G-25和G-100两种凝胶的色谱柱系统的分离范围可从分子量1000左右到数万,几乎包括了水生腐殖酸分子量分布的整个区段。这样的串联凝胶色谱柱的标定曲线为一折线,可用分段函数近似地加以描述。更多还原

【Abstract】 A sequential gel filtration chromatography coupled with UV detector was applied for the characterization of molecular size distribution of aquatic humic substances.The working range of the system using Sephadex G-25 and G-100 covers almost the whole range of the molecular size distribution of aquatic humic substances.The system was calibrated using standard proteins and a discontinuous function can fit the calibration curve well.更多还原