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兔病毒性出血症的诊断及血凝试验方法的比较
DIAGNOSTIC REPORT ON RABBIT VIRAL HEMORRHAGE AND COMPARATIVE STUDIES OF HA METHODS
【摘要】 1990年初,遵义市某养兔场发生一种烈性传染病,兔死亡率达58.7%。通过对12只死兔的病理剖检和实验室诊断,确诊为兔病毒性出血症(俗称兔瘟)。本文报告了用两种不同方法处理的材料进行血凝试验效果的比较,结果经水浴冻融处理后的病料其血凝价没有显著的变化,但可以消除正常兔肝对照材料的非特异性凝集反应。对人工感染死亡兔各脏器含毒价的测定表明,肝、脾的含毒价最高,其次是肺,心的含毒价较低,肾的变动范围较大。在制备兔病毒性出血症灭活苗时,肝、脾、肺为最佳选择材料。
【Abstract】 On infectious disease brake out in a rabbit farm at Zhun yi in the early of February 1990.122rabbits died of the disease within 4 days. the mortality reached 58.7%. 12 dead rabbits were examined pathologically serologically and aetiologically. The disease was definitely diagnosed as rabbit viral hemorrhage. Comparative studies were conducted to obseave the effects of different treatment for materials. The hemorrhage agglutination (HA) titres had no great changes when detecting materials were treated with watev bath. freeging and thawing. But the non - specific agglutination reaction of nornal rabbit liver extracts could be eliminated. The HA titres of different visceral organs were determined in rabbits died from artificial infection. The results showed that liver, spleea and lung had high HA titres heart had low titres and kidney varied with individuals. When lethal vacicnes were prepared liver, spleen and lung would be the first selecting materials.
【Key words】 rabbit viral hemorrhage; hemorrhage agglutination (HA) litre; water bath; freeging and thawing; lethal vaccine;
- 【文献出处】 贵州农业科学 ,Guizhou Agricultural Sciences , 编辑部邮箱 ,1990年05期
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