【摘要】 醋炔诺酮肟通过LFZ—1型旋转薄层仪分离,吸附剂采用硅胶GF₂₅₄,以石油醚:醋酸乙酯（7:3）为溶剂系统,分别得到顺、反式异构体,重结晶后得纯品,测得其各理化参数。从H-NMR图谱分析,顺、反异构体特征吸收峰分别为δ6.54与δ5.84。醋炔诺酮肟在干燥条件下贮藏,或经C₀⁶⁰2.5×10⁴Gy辐照,其中顺、反异构体比例不变。醋炔诺酮肟、顺、反异构体三者配成不同浓度混悬剂,经皮下注射后,比较其抑制Wistar大鼠动情周期天数,实验结果表明,小剂量时三者无区别;剂量8mg/只时,混合体与顺式异构体分别为45.7±2.6天与34.2±6.9天;16mg/时顺式为46.0±2.6天,反式为39.0±4.0天（P<0.01）。抗着床实验结果,顺式、混合体、反式的ED₅₀分别为0.53（0.40～0.64）、0.75（0.69～0.87）与0.97（0.73～1.16）mg/kg更多还原

【Abstract】 Norethisterone Acetate Oxime （NAO） was separated into syn- and anti-isomers by rotable TLC, with silica gel GF254 as absorkent and a mixture solvent of petroleum ether and ethyl acetate （7:3） as solvent. Their physicochemical parameters were determined as well. It was seen that on the H-NMR spectra the vinyl proton of the anti-isomer is relatively shielded by the OH-proton and resonated at higher filed （δ5.84） than the synisomer did （δ6.54）. Moreover, it was found the ratio of syn- and anti- isomers of NAOwas kept constant when the samples were exposed to 2.5×10⁴Gy Co⁶⁰ irradiation source.We studied the biological activities of the above chemicals in suspension. The duration of estrus suppression of syn- and anti-isomers were 46.0±2.6 and 39.0±4.0 days（P<0.01） respectively at the dose of 16 mg per rat. The anti-implantation effect of NAO, syn- and anti- ismoers are effective, with their ED₅₀ relatively at 0.78 （0.69～0.87）, 0.53 （0.42～0.64） and 0.95 （0.73～1.16） mg/kg.更多还原