【摘要】 本文对5’-肌苷酸产生菌的选育及发酵条件对产酸的影响作了研究。 以谷氨酸棒杆菌265为出发菌,利用紫外线硫酸二乙酯以及亚硝基胍等常规诱变手段进行了反复诱变。在此基础上,又采用原生质体诱变的新技术,最后获得一株突变株519,在最佳发酵条件下积累肌苷酸量达7g/L,比原菌株提高了2倍。 本文还对影响发酵产酸的各种因素进行了研究,主要包括种子培养基和发酵培养基、pH值、种龄、接种量、通风量、温度以及添加某些成分等对发酵产酸的影响,并就其中某些因素的影响从理论上进行了探讨。更多还原

【Abstract】 The present article studied on the breeding of 5’-Inosinic acid-producing (IMP) strains and the effects of fermentation conditions on the accumulation of 5’-IMP.Parent strain, corynebacterium glutamicus 265 was step-wise mutated with UV, DES NTG etc.Based on these conventional mutation, a new method-treating protoplast with mutagen-was used. At last, a new 5’-IMP-producing mutant 519, which accumulates 7g of 5’-IMP per liter, two times more than parent strain 265 under the optimal fermentation conditions, was obtained.The factors affecting the accumulation of 5’-IMP have also been investigated, these factors mainly include, seed and fermentation media, pH value, the time of seed cultivation, the amount of seed added to fermentation medium, aeration, fermentation temperature and additions of same substances, etc. The effecting mechanisms of some factors on accumulation of 5’-IMP were discussed in term of theory.更多还原