【摘要】 用脱色苯胺兰诱导荧光的方法观察了蚕豆大孢子发生期间细胞壁胼胝质的变化,结果如下:1.MMC发育早期,其细胞壁上未见骈胝质积累;粗线期以后,合点端细胞壁首先出现尖帽状荧光区。不久,珠孔端也出现亮而钝圆的荧光区。2.大孢子二分体及四分体时期。其间横壁的荧光特亮。3.四分孢子间的三道横壁所发出的荧光总是近珠孔者最弱,其余二道横壁的荧光强度则有不同情况。4.未曾观察到胼胝质完全包围MMC。更多还原

【Abstract】 Observations on behavior of callose deposition in the cell walls during megasporogenesis in broad beau have been made using the method of leuco aniline blue-induced fluorescence. The results are summarized as follows: (1) Callose was absent in the wall of megasporocyte at early prophase. Fluorescence indicating the presence of callose first appeared in the wall of chalazal pole of megasporocyte at pachytene stage. Soon after it, the cap-shaped wall of megasporocyte at micropylar pole exhibited distinct fluorescence. (2) The newly formed transverse walls separating the dyad or tetrad cells fluoresced more intensively. (3) Among the three callosed wails separating the tetradcells, the wall between the two micropylar megaspores often showed weaker fluorescence than the two others. The difference of fluorescent intensity may present between the two others. (4) There was almost on shining in the side walls even though strong fluorescence appeared in the walls located between the megaspores.更多还原