【摘要】 研究用无机物载体(活性炭、分子筛、硅胶)表面吸附固定植物酶(菠萝酶,木瓜酶)技术,以及固定化酶的活性寿命及其催化效能。将实验制得的固定化酶多次反复应用于催化胶液水解反应中。反应结果证实表面吸附固定酶在技术上是可行的,经济上是合理的。另外还采用“补酶”的方法,使吸附酶趋向平衡,以减少该固定化酶中酶的脱落。初步探讨了用与水不溶支撑体的共价结合法固定酶技术。更多还原

【Abstract】 Immobilization technique of enzymes has been studied. Experiments were carried out using porous solid inorganic carriers (activated carbon, molecular sieve , silica gel ) to adsorb the plant enzymes ( bromelain , papain ) . The activity and long-term stability of the immobilized enzymes and desorption of enzymes from inorganic carriers were investigated. In order to examine the long-term stability and catalytic efficiency of the immobilized enzymes continuous runs with immobilized enzymes were made in the hydrolytic reaction. The identification of the behavious of hydrolytic gel verified that the adsorption of enzymes on inorganic carriers to immobilize enzymes is an effective measure. For improving the desorption of enzymes from the immobilized enzymes this work presents compensating enzymes to aqueous solution to impel adsorption of enzymes on inorganic carriers in equilibrum condition. The method of covalently, binding of the enzyme to a Polymer matrix absorbed on inorganic support material is also reported.更多还原