【摘要】 <正> 作为检测DNA单链断裂的一种新方法——碱性洗脱法,首先是由Kohn等报道,随后不少学者改进了方法,并用来进行化学致癌物引起的各种体内、外DNA损伤和修复的检测和研究。但是,利用整体动物体内处理的碱性洗脱法来快速检出环境化学致癌物,国内尚未见报道。为此,我们试用国产微孔滤膜和自己合成的3,5-二氨基苯甲酸盐化合物(简称DABA),同时用几种代表不同化学结构的间接致癌物作为方法学的验证,以图建立小鼠体更多还原

【Abstract】 This paper discribes a DNA alkaline elution procedure to detect DNA damage when mice were treated with chemical carcinogens in vivo. The experiment results showed thatfive carcinogens in divers structure classes including dimethylnitrosamine, 1,2-dimethyl-hydrazine hydrochloride, 4-nitroquinoline 1-oxide, 3, 4-benzo (a) pyrene, 2-acetylamino-fluorene were able to increase the elution rate with statistic significance. Though in theseexperiments home made filter and DABA were adapted, proteinase K was not used. Theexperiments in vivo did yield satisfactory results and reproduceable results were observed.更多还原