【摘要】 本文应用人血栓调节蛋白进行蛋白C功能检测,观察蛋白C在体外活化过程中血栓调节蛋白、蛋白C和凝血酶三者间的相互关系。人血栓调节蛋白在体外与凝血酶形成复合物而加速蛋白C的活化,牛凝血酶比人凝血酶对人血栓调节蛋白有更强的亲和性。血栓调节蛋白与凝血酶在蛋白C活化的作用中均呈现饱和性。本文就钙离子和肝素对体外蛋白C活化的影响及其生理意义进行了初步探讨。更多还原

【Abstract】 Thrombomodulin has been identified to be able to accelerate protein C activation. This paper reports its action affected by related factors in vitro. Different changes in protein C activation mediated by thrombomodulin has been observed in this study, such as different mixture of protein C section, different species of thrombin, different concentration of thrombomodulin, etc. The actions of calcium ions and heparin have been examined also. According to this study we made to measure the effect of variable concentration of thrombomodulin with respect to thrombin and vice verse, the maximun protein C activity seems to be obtained by a 1:1 stoichiometric complex of thrombin and thrombomodulin. The functions and clinical significance about the above related factors need further research.更多还原