【摘要】 用硫酸铵分段沉淀法将华支睾吸虫成虫粗抗原分离成为四种成分。以五种抗原制品(一种粗抗原制品和四种部分提纯抗原制品A、B、C和D)作为抗原,用微量酶联吸附试验(ELISA)检测华支睾吸虫病患者血清中相应抗体的存在。实验表明在五种抗原制品中,以抗原A在诊断华支睾吸虫病方面具有最高的阳性率,患者血清与正常人血清OD值的比值也最高。更多还原

【Abstract】 1. Beijing Institute of Tropical Medicine, Beijing Friendship Hospital 2. Department of Immunology, Institute of Clinical Medical Sciences, China-Japan Frendship Hospital 3. First People’s Hospital of Fuoshan City, Guangdong Province 4. Epidemic Prevention Centre of Fuoshan City, Guangdong Province The proteins of Clonorchis sinensis were fractionated into 4 fractions by a physicochemical method. ELISA tests were performed by using these 5 preparations(1 crude preparation and its 4 fractions, i.e. A,B, C, and D)as antigens to determine the presence of corresponding antibodies in the sera of proved cases of clonorchiasis, paragonimiasis, as well as normal adults. Our study shows that fraction A of clonorchis sinensis has the highest value of the positive rates in the diagnosis of clonorchiasis, and OD ratios of clonorchiasis patients to normal persons among 5 preparations. Therefore,partially purified antigen fraction A of clonorchis sinensis is significantly better than crude preparation both in sensitivity and specificity for the diagnostic purpose.更多还原