【摘要】 本文采用放射性同位素标记淋巴细胞和淋巴母细胞,并结合γ计数、液体闪烁计数测量及放射自显影技术,研究了⁶⁰Coγ射线对Wistar大鼠脾淋巴细胞及ICR小鼠肠系膜淋巴结（MLN）淋巴母细胞在体内移行和归巢的规律和辐射效应,并比较了小鼠MLN淋巴母细胞及外周淋巴结（PLN）淋巴母细胞不同的归巢特点。结果表明:①受照大鼠脾淋巴细胞异常地堆积在肝、肺和脾脏,而归巢到MLN及回肠的数量明显减少;②与PLN相比,来自MLN的淋巴母细胞有选择性地归巢到肠粘膜及与肠道相关淋巴组织的能力;③⁶⁰Coγ线对淋巴细胞及淋巴母细胞在体内移行和归巢的辐射效应与照射剂量有关。小剂量照射对淋巴细胞、中等剂量以上对淋巴母细胞的选择性归巢有明显的抑制作用;④组织切片的放射自显影提示:受照大鼠脾淋巴细胞迁移进入脾脏和MLN的特定区域的能力明显降低,在B细胞区标记细胞数量的减少比T细胞区更为显著。更多还原

【Abstract】 In vitro 6lCo-Y ray irradiation of 5lCr or 3H-UR-labeled lymphocytes from rat spleen and ’ H-TdR-labeled lymphoblasts from mouse mesenteric lympho nodi (MLN) was found to alter their subsequent in vivo distribution significantly in syngeneic rats and mice using techniques of Y-counting and liquid scintillation counting in combination with autoradiography. The experimental results suggested as follows. Irradiated lymphocytes demonstrated an increased distribution to the liver, lungs and spleen. Cells going to the MLN and gut-associated lymph-oid tissues (GALT) showed a significant decrease in homing following irradiation. The effects of e’Co-Y rays on the lymphocyte and lymphoblast traffic and homing were related to the radiation doses. There was a significant inhibiting effect on the ability of selective homing of MLN lymphoblasts to GALT and intestinal mucosa with doses over 4 Gy, while the selective homing of splenic lymphocytes was affected after 0.5 Gy exposure. The autoradiography showed that the migration of the irradiated lymphocytes into B cell areas of MLN and spleen was depressed more remarkably than that into T cell areas. These studies. provide some experimental materials for radiation immunology.更多还原