【摘要】 <正> 黄单胞菌是目前用于生产细菌多糖的优良菌种。它是一种好气性细菌,在发酵生产时受溶解氧的影响较大,但它的溶氧要求,未见详细报道。在发酵生产高粘度的产品时,通用式机械搅拌发酵罐很不适用,国外在改进高粘度生物反应器方面已做了不少工作,但多数涉及商业性生产问题,未曾公开发表。我国近年来也开始研制适用于高粘度的生物反应器,其中的关键问题之一是如何增进发酵液的溶解氧。因此,研究黄单胞菌对溶氧的要求,是研制新型反应器所必不可少的基础工作。本文就此进行了试验。更多还原

【Abstract】 When the polysaccharides were produced by Xanthomonas campestris S-152,the demand of dissolved oxygen has been studied.The productivity of polysaccharides,the correlation of viscosity and shear rate,the content of pyruvicacid and the viscosity of culture broth under different K_d value are tested.Experiments show that the quality of polysaeeharides is delined,if the Kdvalue is less than 1.828×10^-7,and the quality is normal where the K_d valueis in the range 2.063—2.587×10^-7.更多还原