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L-d树脂制备溶菌酶的工艺研究

A Novel Route for the Preparation of Lysozyme by L-d Absorbent

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【作者】 李吉高; 吴蓉蓉;

【Author】 Li Jigao Wu Rongrong (Nanjing Institute of Biochemical Pharmacy)

【机构】 南京生物化学制药研究所; 南京生物化学制药研究所;

【摘要】 本文报导溶菌酶生产新工艺,针对国内原工艺存在的问题,成功地研究了以离子交换与吸附相结合的机理制备溶菌酶新方法。具有专一性吸附好,工艺简单的特点;革掉了盐析、透析、冻干的工序;节约了大量的动力消耗,化工原料,生产周期从原来7天缩到2~3天;产品活力高,成本低,适合蛋品联产工业化条件。产品酶活力每毫克15000单位,最高20000单位(原工艺9000单位),得率每克蛋清50000单位(原工艺30000单位)。

【Abstract】 The conventional lysozyme production gives several problems and low yields. On the contrary, lysozyme is easily prepared by using L-d absorbent which is based on the principle of combaining ion-exchange with absorbtion. Since the main steps -solting out, dialysis and freeze drying-may be cut down, the working process is simplified and the cycle time is shortened from 7 to 2-3 days. A high yield (-50000u/g egg white) and high aclivities (15,000-20,000u/mg) can be obtained by using the new route at Iow cost (30000u/g and 9000u/mg by conventional way) . In addition, it provides means for multiple utilization of the egg products.

  • 【文献出处】 离子交换与吸附 ,Ion Exchange and Adsorption , 编辑部邮箱 ,1988年04期
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