【摘要】 液氮内保存的FCC—1株恶性疟原虫红内期用蜡烛缸—平皿法体外连续培养40天。疟原虫复苏后培养的第3天原虫密度开始上升.第11天达到最高峰,其中一皿的红细胞含虫率高达20.56%。影响恶性疟原虫体外培养的有关因素如RPMI—1640培养液,血清和红细胞,本文进行了讨论。更多还原

【Abstract】 FCC--1 strain P. falciparum preserved in liquid nitrogen had been culturedcontinuously for 40 days by using glass petri dishes in a candle jar. The resultsindicate that, on the third day of cultivation after the cryopreserved parasitizedblood was thawed, the parasite density began to rise gradually, and on the eleve-nth day,the density rose to the highest level: in one of the Petri dishes, the rate ofparasited red blood cells amounted to 20.56%, Several factors affecting thecontinuous cultvation in vitro of P. falciparum, such as RPMI--1640medium, serum and red blood cell using for cultivation are. discussed in thisarticle.更多还原