【摘要】 为了提高时间相关单光子计数方法测量荧光寿命的准确性,本工作使用SP—70纳秒荧光光谱仪,系统地研究了影响测量准确性的因素.仪器响应函数P(t)主要取决于停止光电倍增管的响应函数H(t)和激发光源的响应函数L(t),响应函数H(t)的畸变主要取决于停止光电倍增管的电压分布,闪光灯上所加激发电压,充气气压,以及灯电极间隙对激发光源的响应函数L(t),有着不可忽略的影响.为此,我们在实验中摸索出了解决办法,给出了仪器最佳实验条件.更多还原

【Abstract】 In order to improve the measaring ac-curacy of short-lived fluorescence decayusing single photon counting technique,a SP-70 nanosecond spectrometer is usedto study systematically the factors whichaffect measurement accuracy.The instru-ment-response-function P(t) depends main-ly on the response function H(t) of stop-photomultiplier and the response functionL(t) of excitation source.The distortionof the response function H(t) is stronglyrelated to transit time of stopphotomu-ltiplier.In addition,the excitation volt-age,gas pressure and electrode gap offlash lamp have unnegligible effects on theresponse function L (t) of excitationsource.The optimum experiment conditions of the instrument are given.更多还原