【摘要】 IB-RS-2细胞经秋水仙素(2微克/毫升)处理24—48小时,约75％的细胞形成微核,同时在细胞表面形成许多大小不一的原生质突起。微核化的细胞进一步用松胞素B(10微克/毫升)处理,在Ficoll不连续梯度(12.5％、16％、25％)中进行离心去核、形成许多微细胞。扫描电镜观察表明,微细胞是微核化细胞的原生质突起脱落而形成的。微细胞是一个不均一的群体,无论在大小与表面形态,还是在微细胞内部的成分与结构上都存在很大差异。更多还原

【Abstract】 IB-RS-2cells were treated with colchicine (2μg/ml) for 24-48 hours. Seventy-five percentage of the cells formed micronucleated cells, meanwhile, many peotoplasmic spherical buds appeared on the surfacce of the cells. The buds were different in size and shape. The larger plasma buds were about 5-10 μm in diameter. There were many microvilli on the surface of some larger buds (Plate 1. Fig. 1, 4), while the others had hardly any microovilli on their surface. The smaller buds were about 1-2 μm in diameter, with smooth surface.The micronucleated cells were treated with cytochalasin B (10μg/ml), then were centri-fuged within discontinuous I icoll 400 gradients for enucleation. The plasma spherical buds were broken off from the "parent cells", thus microcells were produced. These microcells were like the plasma buds on the surface of micronucleated cells. The microcells were much different in size and shape. The larger microcells were 5-7 μm in diameter. Some of them had many microvilli, and others had not. The smaller microcells were about 1-2 μm, and all of them were smooth on their surface.The microcells can be divided into two classes. The first class microcells contained chromosome, or chromatin, and the second ones did not. Some microcells were lysed and degenerated, with only membrane left. The microcells are a hetergeneous population. There are much difference in size and shape of the microcells as well as in the composition and structure of them.更多还原