【摘要】 应用光生物素（Photobiotin）标记大麦黄矮病毒（BYDV）cDNA分子杂交探针,可用于检测大麦黄矮病毒。反应灵敏度至少可达1ng。在灵敏度相同的情况下,与常规应用α-³²p-dCTP标记的分子探针相比,具有安全、稳定、方便、经济的优点。更多还原

【Abstract】 cDNA fragments from barley yellow dwarf virus ( BYDV ) were cloned and one cDNA clone was used as hybridization probe. Dot blot assays using photobiotin-labeled plasmid probe successfully detected the BYDV plant extract samples and the sensitivity was at least l ng. Comparison of dot blot hybridization between photobiotin labeled and α-32P-dCTP labeled pro-bes for the detection of BYDV, showed that photobiotin labeled one was su-perior in safety, stability, convenience and economy.更多还原