【摘要】 用以激活环核苷酸磷酸二酯酶活性为基础的钙调蛋白(Calmodulin CaM)活性测定法,测定正常兔红细胞及兔网织红细胞CaM含量及阿片肽类对兔网织红细胞膜CaM活性的影响。发现无论在网织细胞或其细胞膜CaM含量均明显高于正常红细胞;β-内啡肽和强啡肽对兔网织细胞膜CaM活性有显著的抑制作用,这种作用并不为纳洛酮或其化合物Mr2266所拮抗,也不受钙离子浓度增加的影响,但可被外源性CaM所翻转。推论兔网织细胞膜CaM是β-内啡肽和强啡肽作用环节之一.更多还原

【Abstract】 Calmodulin （CaM） content in rabbit reticulocyte and influence of opioid peptides on CaM activity in its membrane -were studied by a highly sensitive assay based on stimulation of Calcium-dependent phosphodiesterase activity. The CaM content in reticulocyte was higher than that in normal erythrocyte both in cytosol fraction and its membrane. Among them, α-endorphin （α-EP） and dynorphin 1-13（Dyn） showed significant inhibitory effect on CaM activity in reticulocyte membrane in capable of being antagonized by nalo-xore,Mr2266 or increase of Ca²⁺ concentration,except for reversion due toexogenous CaM,suggesting of effect of β-EP and dyn on reticulocyte mem-brahe as a result of nonopioid mechanism with CaM as important linkage.更多还原