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检定稻田天敌与害虫捕食关系的新方法——炭凝集试验
A New Method to Assay the Predatory Relation of Natural Enemy and Pest in the Paddy Field——Carbon Agglutintion Test
【摘要】 <正> 炭凝集试验在流行病学上,如对鼠疫、霍乱、伤寒、痢疾、钩端螺旋体等疾病的诊断和鉴别方面,在国内外已有广泛的研究与应用;在植病的检验上,也作为一种检测方法.利用羊的红血细胞和聚苯乙烯胶状乳颗粒作为载体,用于田间检定捕食者与被捕食者的关系已有文献报道.本文利用炭粉作为载体的凝集反应方法,用于检测捕食者与被捕食者的关系.
【Abstract】 The method of carbon agglutintion test (CAT) was used to investigate the relatoin of the wolf spider and its prey, rice leafroller, Cnephalocrocis medinalis and was fund that the rate of positive reaction of CAT was 91.49% while the rate positive reaction of double diffusion test was 70.21% in 47 samples, the sensitivity of CAT has increased 128 times than positive reaction of double diffusion test, and cut short test time from 24-48 hrs to 1 hr.The method included preparation of antigen of prey, preparation of antiserum, purification of antibody, preparation of carbon antiserum, collection and preparation of predator and carbon agglutiotion test.
【Key words】 Natural enemy; Pest; Predatory relation; Method to assay; Carbon agglutintion test;
- 【文献出处】 中山大学学报(自然科学版) ,Acta Scifntiarum Naturalium Universitatis Sunyaatseni , 编辑部邮箱 ,1987年03期
- 【被引频次】1
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