【摘要】 应用放射免疫和免疫组织化学法观察到大鼠心房内存在心钠素前体原56-92样免疫活性物质,这种物质可能分泌入血,分子量约为6,000道尔顿。初步研究发现人工合成的心钠素前体原56-92可以抑制由去甲肾上腺素引起的家兔主动脉收缩。它可能是心钠素前体分子裂解产生的一种激素。更多还原

【Abstract】 Prepro atrial natriuretic factor 56-92 (prepro ANF56-92) -like immunoreactive material was found in rat atrium (535±55 fmol/mg protein) and plasma (40 find/ml) by radioimmunoassay. PAP-immunohistochemical study demonstrated that specific staining of granules for prepro ANF 56-92 were located around the nucleus of atrial cardiocytes. Ge) flltration and reverse-phase HPLC analyses revealed that this prepro ANF 56-92-like immunoreactive material in rat atrium with a molecular weight about 6,000 daltons differed either from prepro ANF 56-92 or prepro ANF 26-92. Bioassay study of rabbit aortic strip in vitro showed that prepro ANF 56-92 can inhibit norepinephrine-induced aortic contraction. These results suggested that this preproANF56-92 -like immunoreactive materia may be a novel hormone candidate released by rat atrium.更多还原