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含酚棉籽饼对公兔抗精子发生效应的研究
EFFECTS OF COTTONSEED CAKE CONTAINING GOSSYPOL ON ANTISPERMATOGENESIS OF MALE RABBIT
【摘要】 以占日粮不同比例的含酚棉籽饼喂饲公兔后,观察了睾丸曲细精管的组织学变化,以探讨含酚棉籽饼对公兔的抗精子发生效应和饲用方法。当棉籽饼占日粮的5%、喂饲20天时,公兔睾丸生精上皮正常,能产生形态正常的精子;同样的比例、喂饲40天,或棉籽饼在日粮中超过10%喂饲20天,均可导致睾丸生精上皮蜕化,精子形成受阻。试验表明,以含酚棉籽饼喂饲公兔的适宜量, 40天内不应超过日粮的10%,60天内不应超过日粮的5%。睾丸生精上皮受损时并无因进食含酚棉籽饼而出现的临床中毒症状。同时观察到血清丙谷转氨酶(SGPT)在任何添加比例下,20天时均有升高,无随喂饲时间延长进一步升高的趋向。
【Abstract】 It was demonstrated that including cottonseed cake ( contained 0.137% free gossypol ) in different proportions in male rabbit diet resulted in histological degeneration of seminiferous epithelium. The degree of degeneration positively correlater with the percentages of cottonseed cake included in the diet and the duration of feeding. Before appearing the gossypol poisoning symptoms clinically the effect of antispermatog-enesis has been shown histologically. No histological changes wore observed in liver, kidney and spleen.Meanwhile the serum glutamic-pyruvic transaminase ( SGPT ) rose remarkbly at any proportions included. The experiment shows that continuous feeding 5 % cottonseed cake containing gossypol in the diet over 20 days is not suggested. It was postulated that the diagnostic standard for animals poisoned by cottonseed cake might should be revaluated.
- 【文献出处】 畜牧兽医学报 ,Chinese Journal of Animal and Veterinary Sciences , 编辑部邮箱 ,1987年01期
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