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赤松毛虫质型多角体病毒血清学初步研究

A SEROLOGICAL STUDY ON VIRIONS FROM THE CYTOPLASMIC POLYHEDROSIS VIRUS (CPV) OF PINE MOTH DENDROLIMUS SPECT ABILIS BUTLER

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【作者】 王志贤高志和陈昌洁

【Author】 Wang Zhixian Gao Zhihe Chen Changjie (Forest Research Institute, Chinese Academy of Forestry)

【机构】 中国林业科学研究院林业研究所中国林业科学研究院林业研究所

【摘要】 <正> 日本赤松毛虫Dendrolimus spectabilis CPV已经作为一种病毒制剂在日本被批准登记注册使用(Aizawa,1976)。该病原对我国台湾省马尾松毛虫D. punctatus的感染也获得成功(Ying Sze-Ling 1970)。自1982年以来。我们引用该病毒先后对我国的赤松毛虫D. spectabilis、油松毛虫D. tabulaformis、马尾松毛虫D. punctatus、文山松

【Abstract】 Experiments proved that the pine moth Dendrolimus spectabilis, D. tabulaeformis, D. punctatus, D. punctatus Wenshanensis and cotton Bollworm Heliothis armigera and silkworm Bombyx mori could be infected by D. spectabilis CPV which was introduced from Japan.The antigens in this test were from D. punctatus which was infected by D. spectabilis CPV from Japan and other pine moths, these antigens were purified by sucrose gradient centrifugation.Antiserum was obtained by immunizing rabbits with purified preparations of virions from CPV of D. punctatus (infected by Japanse D. spectabilis CPV).Serological comparision of virions from above mentioned pine moths was done by absorption test and counter immuno electrophoresis. The results obtained are as follows:The results of the experiments indicated that all pine moth CPVs,no matter whether it is Chinese or Japanese were serologically indistingui shable.Antiserum of pine moth CPV virions did not cross-react with three NPV and one GV.The pine moth CPV and cotton bollworm CPV were found to be serologically closely related, but the pine moth CPV and silkworm CPV could be differentiated using absorption test and counter immuno eletrophoresis technique.

  • 【文献出处】 林业科学 ,Scientia Silvae Sinicae , 编辑部邮箱 ,1987年02期
  • 【被引频次】4
  • 【下载频次】12
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