【摘要】 本文报告理化因素对抗人N单克隆抗体（ON₁D₃）活性的影响。P^H及反应温度对ON₁D₃单克隆抗体与人红细胞的血凝反应有显著的影响。较适宜的实验条件为P^H7.0,温度30℃。在此条件下对155例新鲜血液及60例陈旧血痕样品进行MN分型。结果与用市售兔抗N血清测定的结果完全一致,证明在上述条件下,ON₁D₃单克隆抗体可用于临床常规血液分型及法医学上对陈旧血痕MN血型的测定。更多还原

【Abstract】 The effects of physicochemical factors on hemeagglutination reaction of monoclonal anti-N antibody（ON₁D₃）were investigated.Under precise physi- cochemical conditions monoclonal anti-N can be used as blood typing re- agents both in clinical blood test as well as blood stains.It was demonstrated that both the pH and the temperature have an obvious effect on the agglu- tination reaction.The best condition for agglutination reaction is pH7.0, 30℃.Under these conditions ON₁D₃ McAb was used,in parallel with poly- clonal rabbit antisera,for N typing of 155 random blood donors and 60 old blood stains.The results obtained were in perfect agreement更多还原