【摘要】 测定培养温度、琼脂浓度、感染系数、培养基丰度和时间等因子对形成噬菌斑大小的影响程度,从而排除了影响噬菌斑大小的非遗传本底。分析电镜下噬菌体颗粒大小和平板上噬菌斑大小两者之间的关系,发现在同一容量较恒定的细胞内,不向噬菌体其生物合成总量趋于相等,该原理解释了决定噬菌斑大小的主要遗传控制方式。计算并推导出在一定程度上可反映噬菌体颗粒大小的经验公式。更多还原

【Abstract】 In order to find out the interrelationship between the diameter of plaques and the size of bacteriophage particles, this paper tested temperature, concentration of agar, concentration of host cells, concentration of medium, cultivation time etc. for their influence on the size of plaques formed by T4 and φX174. After exclusion of non-hereditAry factors, we discovered that the total quantity of biosynthisis is equal in various phages in the same host cells, that is, the diameter of plaques shows inverse rela-tion to the size of particles. An empirical formula was deduced from the experimental data: We can estimate the size of bacteriophage particles by observing the size of plaques using this formula.The main reasons why difference in plaque size exists, and the reason why Ml3, λ charon 4A etc. do not fall in with this formula are discussed.更多还原