【摘要】 鸭血清HDL用DS和CaCl₂沉淀浓缩,再经角头密度梯度超速离心纯化。纯化的HDL经低温下脱脂得到apo HDL。然后经过Sephadex G-200柱层析和DEAE-纤维素离子交换柱层析得到apo CⅡ。后者经PAGE和SDS-PAGE鉴定为纯品。所获HDL-apo CⅡ可有效地激活LPL酶。更多还原

【Abstract】 Apo C Ⅱ is one of the most important apolipoproteins. It can activate lipoprotein lipase which catalyzes hydrolysis of triglycerides in lipoprotein particles, so it plays an important role in metabolism of lipoproteins and triglycerides. Besides,the level of HDL correlates negatively to incidence of atherosclerosis. Basing on the reasons above, it would be profitable to study duck serum HDL and HDL-apo CⅡ.Pure duck serum HDL was prepared by dextran sulfate precipitation and ultracentrifugation in a density gradient. Apo HDL was obtained by deli-pidation with ethanol-ether at -10℃ and then duck HDL-apo CⅡ isolated on Sephadex G-200 and gel DEAE-cellulose ion exchange columns. Purity of HDL-apo C Ⅱ was identified with PAGE and SDS-PAGE to have only one band.更多还原