【摘要】 <正> 前列环素（FGI₂,其稳定代谢产物6-keto-PGF₁α）和血栓素A₂（TXA₂,其稳定代谢产物TXB₂）为花生四烯酸代谢途径的两个重要产物,它们的生理活性恰巧相反,前者抑制血小板聚集和扩张血管,后者诱导血小板聚集和收缩血管。二者平衡如果失调,可能导致某些疾病的发生。为了推广敏感的、特异的测定6-keto-PGF₁α和TXB₂放射免疫法,我们用Na[¹²⁵I]成功地标记了6-keto-PGF1α和TXB₂。更多还原

【Abstract】 Na [125I] -labeled antigens with high specific activity were prepared by radioiodination of 6-Keto-PGF1a or thromboxaneB2-tyrosine methyl ester conjugate followed by purification with thin-layer Chromatography. Using Na [125I] 6-Keto-PGF1a-TME or Na[125I] TXB2-TME as labeled antigens to develop radioimmunoassay which offer some advantages such as high specific activity, low bound-to-total ratio in the absence of antibody, simple handly and particularly economical when a lot of simples assayed更多还原