【摘要】 制成了亲和力高、特异性强的P物质抗血清,并建立了灵敏度高、特异性强、稳定性好的P物质放射免疫分析法。最小检出量为2～4fm/管,最适测定范围为12.5～400fm/管,适用于生理学、神经生物学、生物化学和药理学等领域的研究工作。更多还原

【Abstract】 Radioimmunoassay for SP was reported. Synthetic SP was coupled to suc~ cinylated thyroglobulin by 1-ethyl-3- ( 3 dimethylaminopropyl) carbodiimide. The SP-thyroglobulin conjugate was used for immunization of rabbit. The Ka value of antiserum from rabbit No XI is 2.0×1010L/M. The antibody was used at a final dilution of 1:30,000, and cross-reactivities estimated were: eledoisin 0.1%,eledoisin-related-peptide 0.008%, bombesin 0.2%, physalaemin 33~59%. No cross-reactivity with ACTH, angiotensin Ⅱ and met-enkephalin. Iodination of tyr8-SP was performed with chloramine T method and separated with sulf-opropyl sephadex C 25 column. SP radioimmunoassay was thus developed. The minimum detectable amount in this assay is 2~4 fm per tube. The recovery is essentially quantitive. Coefficients of variation ( CV ) within and between assay were 5.6 and 11% respectively.更多还原