【摘要】 应用比较简单的一次四氯化碳澄清,二次聚乙二酵浓缩沉淀,差速离心和20％—50％的蔗糖密度梯度离心,可以获得分离提纯效果较好的水稻齿叶病病毒（RRSV）制剂,在260nm处有最大吸收值,在A₂₆₀/A₂₈₀的比值为1.6。用醋酸铀负染方法,可以观察到RRSV为直径54nm—65nm的球状颗粒,具有底部较宽的突起,突起的高度为8—11nm,宽度约为22nm。病毒的核酸为双链RNA,共有十组分散的基因组,这一结果和四方报道的一致,在这一提纯病毒的制剂中,尚发现有其他三种大小不同的球状和线状颗粒,对它们的可能来源进行了讨论。更多还原

【Abstract】 The partial Purified preparations of Rice Ragged Stunt Virus （RRSV） from fresh diseased rice leaves could be obtained in the high yield by the combination of the techniques of clarification with CCI₄, precipitation with Polyethylene Glycol,differential centrifugations and linear sucrose gradient centrifugations. The preparations showed the maxium of the ultra-violet absorption spectra was at 260 nm and the value of the A₂₆₀/A₂₈₀ ratio was 1.6. By Electron microscopy the RRSV particles showed spiked isometric morphology in diameter ranged 54—65 nm. Electrophoresis experiments have demonstrated that the RRSV contain 10 separated ds-RNA genomes. The sizes and electrophoresis pattern of 10 viral ds-RNA genomes were consistent with the results reported by S. Kawano et al. In the partial purified preparations of RRSV three kinds of other particles could be also observed. Two isometric particles were in diameter about 75nm and 35nm respectively. The third had filamentous appearance and the concentration of the filaments obviously increased with the steps of purification of RRSV The possible origins of these three particles were discussed.更多还原