【摘要】 <正> 在运动医学领域中,由于运动员的关节软骨伤病发生率远高于普通人,同时又缺乏有效的治疗方法,影响训练和运动成绩的提高,因此对关节软骨伤病的研究一直是重点科研项目之一。对关节软骨进行研究的手段近20年来有了巨大进步,其标志是关节软骨细胞在体外分离培养取得成功。它作为探索软骨细胞性状的先进手段正越来越广泛地被应用到关节软骨损伤和疾患的研究工作中。我所1982年在体外分离培养关节软骨细胞获得成功。近年来,对关节软骨细胞在体外培养过程中所更多还原

【Abstract】 Using isolating and culture of articular chond ytes technique, six generations of chondrocytes from one rabbit were gained for experiment. The research methods were used as following: Toluidine blue staining method, morphological observing under inverted contrast microscope, S³⁵ isotope autoradiography and LDH isoenzymes electrophoresis.The results revealed the articular chondrocytes were undergoing dedifferentiation in culturing. The main symbols of articular chondroeytes dedifferentiation were corresponding to following review: 1. chondrocytes changed the polygonal shape to spindle-like; 2. loss of synthetic capability of CSPG and changing the synthetic collagen type Ⅱ to type Ⅰ. Other than those mentioned above, in our experiment, we found the spectrum of LDH isoenzymes in culturing chondrocytes was changing and the proportions of each LDH isoenzyme as well during the culture course （LDH₁ increasing and LDH₃ decreasing）, it implied that the glycolytic pathway of chondrecytes must be changed. The author suggested the change of LDH isoenzymes be considered as one of the symbols of dedifferentiation of articular chondrocytes.It was pointed out that the studies of cartilage biology and cartilage repairing by means of chondrocytes in culture were shackled by dedifferentiation phenomenon. Because the biology properties of cultured chondrocytes were little change from normal articular cartilage within 3 generations, therefore these chondrocytes could be regarded as articular chondrocytes model.It is also proved that the increase in proportion of bovine fetal serum in media neither reversed nor changed the dedifferentiation course of articular chondrocytes.更多还原