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抗人A型红细胞单克隆抗体的杂交瘤细胞建株
AN ANTI-HUMAN GROUPING A RED CELL MONOGLONE ANTIBODY HYBRIDOMA CELL LINE
【摘要】 SRBC-B3单克隆杂交细胞株是用小鼠骨髓瘤细胞 SP2/0与经过 A 型红细胞免疫BALB/C 小鼠脾脏中的 B 淋巴细胞融合而成的细胞株。该株能分泌抗 A 抗体,与 A 型红细胞发生凝集,有高度专一性与特异性。无额外抗体,对 ABO 血型鉴定有一定可靠性。
【Abstract】 SRBC-B3 monoclone hybridema cell line is a clone derived from the fusion ofmouse myeloma cell SP 2/0 with lymphocyte B cell from spleen of BAL B/C mousewhich was immunolized with grouping A red cell.This monoclone hybridoma cell linesecreted anti-A antibody,aggregated with grouping A red cell with high specificity andaffinity.This antibody is not only reliable but also cheap,and it may be used in ABOblood grouping test.
- 【文献出处】 上海医学 ,Shanghai Medical Journal , 编辑部邮箱 ,1986年11期
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