【摘要】 用PEG6000沉淀和密度梯度离心从组织培养病毒感染液和粪便标本中浓缩提纯了人和牛轮状病毒,这有助于低浓度大体积标本的检出。对纯化病毒粒子的理化性质进行了研究,完整病毒粒子直径66nm,浮力密度1.36g/ml,紫外吸收光谱A280/A260=0.63,PAGE分离出MW分别为65,43,32,130,86,25,18×10~3七条结构多肽,单壳粒子浮力密度1.38g/ml,PAGE分离出MW为43,130×10~3两条结构多肽。更多还原

【Abstract】 Rotaviruses were concentrated and purified from large volumes of tissue culturefluids and faecal extracts using PEG 6000 precipitation,centrifugation through 15～40%sucrose and segregation by density in a CsCl density gradient.Electron microscopicexamination of the concentrated preparation revealed typical rotavirus particles.Thepolypeptide components were analyzed by PAGE(SDS-CONT).更多还原