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测定Epstein-Barr病毒IgA/EA抗体的改进方法

AN IMPROVED METHOD FOR DETECTION OF EPSTEIN-BARR VIRUS IgA/EA ANTIBODY

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【作者】 皮国华; 曾毅; 余世荣; 方仲; 赵全璧; De-ThéG.;

【Author】 Pi Guohua Zeng Yi Yu Sherong Fang Zhong Zhao Quanbi De-The G Institute of Virology, Chinese Academy of Preventive Medicine.Institute of Radiomedicine.CNRS, France.

【机构】 中国预防医学科学院病毒学研究所; 中国医学科学院放射医学研究所; 法国国家科学研究中心里昂Alexis Carrel医学院;

【摘要】 用免疫酶法(IE)检查鼻咽癌(NPC)病人血清中IgA/EA抗体,阳性率为73%,几何平均滴度为25,将血清用马抗人IgG血清或葡萄球菌菌体A蛋白(SPA)处理后,以除去竞争性IgG类抗体后,阳性率可增高至92%,几何平均滴度提高到89,有15例NPC病人血清,经马抗人IgG血清处理前IgA/EA抗体为阴性,处理后均呈阳性反应。

【Abstract】 IgA/EA antibodies of Epstien-Barr virus was examined in sera, were treated with anti-human IgG or SPA,by immunoenzymatic test. 92% of 82 NPC patients were positive with a GMT of 1:89 in sera treated with anti-human IgG, while only 73% of untreated sera were positive with a GMT of 1:25. IgA/EA antibody was demonstrated in 15 additional sera from NPC patients after treatment with anti-human IgG. These data indicate that the modified method is more sensitive for the detection of IgA/EA antibodies.

  • 【文献出处】 病毒学报 ,Chinese Journal of Virology , 编辑部邮箱 ,1986年04期
  • 【被引频次】8
  • 【下载频次】24
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