【摘要】 用聚丙烯酰胺凝胶电泳方法分析了流行性感冒病毒重组株京生75-29R2 T1（H3N2）及冷适应株31-广（H3N2）的RNA及多肽。重组株京生75-29R2 T1的HA及M基因系来自流行病毒亲本株/甲/北京/29/75（H3N2）,而P₂、NA、NP及NS基因则来自温度敏感母株福R3（H2N2）。流行病毒株甲/穗/03/68（H3N2）在低温条件下经鸡胚尿囊腔传递24代而获得的冷适应疫苗毒株31-广（H3N2）其基因型与野毒株一致。更多还原

【Abstract】 Virion RNA segments and polypeptides of influenza A virus recombinant ts Jingsheng 75-29R2T1 were analysed by examination of the migration rate of RNA and virus-induced polypeptide in polyacrylamide gel electrophoresis. The results indicated that the HA and M genes of the recombinant originated from the field strain A/Beijing/29/75 ( H3N2 ) ,and the p2, NA, NP and NS genes originated from the ts parent FuR3 ( H2N2 ) .The recombinant virus aquired the ts gene ( NP ) of FuR3 and became attenuated for man.Cold-adapted strain 3l-Guang ( H3N2 ) was passaged 24 times in the allantoic cavity of chick embryo at lower temperature resulting in attenuation for man, but the migration rats of RNAs in PAGE were the same as those of its parental strain A/Sui/03/68 (H3N2).The mutations responsible for cold adaptation thus did not alter the electrophoretic mobility of the RNA segments concerned.更多还原