【摘要】 应用血卟啉衍生物(HpD)作用于人宫颈鳞癌细胞,当剂量为16μg/ml再光照2分钟后,透射电镜下即出现线粒体基质的灶性空化,5分钟后线粒体肿胀,核基质凝聚。光照10分钟再持续作用30分钟,上述改变更加明显,又出现粗面内质网扩张及核肿胀;60分钟后线粒体嵴呈球状嵴消失,内质网呈现空泡样结构,细胞膜裂解。扫描电镜下于光照2分钟后细胞肿大;10分钟后表面绒毛减少,泡状突起增加,有的断裂呈指状突;90分钟细胞裂解成大小不等的团块。作者认这线粒体是HpD光敏作用最早的靶细胞器,其他改变可能是线粒体损伤导致的继发效应。更多还原

【Abstract】 Ultrastructural studies on effect of HpD on carcinoma cell line were reported. A clonal cell line of cervical carcinoma (CC-801) was used. In group of 16UUUUUUUUUg/ml HpD,the matrices of some mitochondria were decreased in density 2 minutes after light exposure and swollen mitochondria with translucent matrices and slightly aggregated nuclear chromatin appeared 5 mins later. 30 mins after irradiation, these degenerative changes became pronounced and cisterna of rough endoplasmic reticulum dilated with swollen nuclei.1 h after irradiation, the swollen mitochondria showed a balloon structure without cristae,endoplasmic reticulum became more dilated and plasma membrane was broken and whole ended with necrosis.SEM revealed that the cells became slightly swollen 2 mins after irradiation, membrane vesicles developed and microvilli reduced in their number 10 mins after irradiation and then the vesicles appeared broken from thtir appendages and only residues remained on the surface.90 mins after irradiation,some cells were broken into debris wlth differents sizes.We suggest that mitochondria may be the earliest target for photosensitizing effect and other cellular changes are the secondary effect.更多还原