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快速染色法检查疟原虫

RAPID STAINING METHODS FOR DETECTION OF MALARIA PARASITES

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【作者】 陆惠民; 孙溱安; 唐学恒; 车惠兰; 谢可鸣; 保泽洛;

【Author】 Lu Huimin, Sun Zhenan, Tang Xueheng, Che Huilan, Xie Keming, Bao ZeluoDepartment of Parasitology, Suzhou Medical College

【机构】 苏州医学院寄生虫学教研室; 苏州医学院寄生虫学教研室;

【摘要】 本研究以乳化剂OP代替费氏染液中的缓冲溶液,改进了厚、薄血膜染色方法,并将染粉及表面活性剂分装于软罐或安瓿内,适于现场和临床疟原虫检查,是一种简便、快速、可靠而价廉的方法,并改进了吉氏快速染色。

【Abstract】 Seven non-ionic surface active agents have been used in place of buffer solution pH 6.8 in Field Stain and the staining methods have been improved. The stains containing these non-ionic surface active agents can be prepared for immediate use due to their solubility. The stains kept in different flexible cans or ampoules are particularly suitable for the application in field research or survey. This staining method for thick films with complete hemolysis is highly applicable in field survey and clinical diagnosis of malaria. Both Schuffner’s or Maurer’s dots are visible in red cells infected with Plasmodium vivax or P. falciparum in thin smears. When 10% Giemsa solution prepared with 1% non-ionic surface active agent is used, the time for staining smears needs only 3 minutes.

  • 【文献出处】 寄生虫学与寄生虫病杂志 ,Chinese Journal of Parasitology and Parasitic Diseases , 编辑部邮箱 ,1985年01期
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