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酶标葡萄球菌A蛋白组化法在流行性出血热诊断上的应用
Use of Indirect Immunoenzymatic Histochemical Method with HRP-SPA for the Diagnosis of EHF
【摘要】 使用辣根过氧化酶标记葡萄球菌A蛋白(HRP—SPA)间接免疫酶组化法检测100例流行性出血热病人、54例非出血热病人及31名正常人血清中出血热异特性抗体。结果表明(HRP—SPA)在灵敏度和特异度上与间接免疫荧光法IFA基本相同,由于HRP—SPA简便易行,可作为EHF诊断的一种新方法。
【Abstract】 An indirect immunoenzymatic histochemical method with horse radish peroxidase-labelled staphylococcus protein A (HRP-SPA) was used to determinate the special EHF-antibody in the serum of 100 patients with EHF, 54 non-EHF patients and 31 normal individuals. The results showed that the method with HRP-SPA is equivalent to the indirect immunofluorescence method (IF) in sensitivity and specificity. Since the indirect immunoenzymatic histochemical method with HRP-SPA is simple and easy, the anthors recommend it as a new method for the diagnosis of EHF.
【关键词】 流行性出血热;
葡萄球菌A蛋白;
免疫荧光;
免疫酶组化法;
【Key words】 epidemic hemorragic fever; staphylococcus protein A; immunofluorescence; immunoenzymatic histochemical method;
【Key words】 epidemic hemorragic fever; staphylococcus protein A; immunofluorescence; immunoenzymatic histochemical method;
- 【文献出处】 徐州医学院学报 ,Acta Academiae Medicinae Xuzhou , 编辑部邮箱 ,1985年04期
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