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应用卵白素-生物素-过氧化物酶复合物法观察大鼠尾壳核、隔核及视前区脑啡肽的定位

USE OF AVIDIN-BIOTIN-COMPLEX IN INVESTIGATION OF ENKEPHALIN LOCALIZATION IN RAT CAUDATEPUTAMEN, SEPTAL NUCLEI AND PREOPTIC AREA

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【作者】 何莲芳; 李宽(女严); 董惟强;

【Author】 HJE LIANFANG, LI KUANYAN, DONG WEIQIANG(Laboratory of NeurophysMogy and Neuromorphology, Research Department of Acupuncture, Faculty of Basic Medical Sciences, Shanghai First Medical College, Shanghai)

【机构】 上海第一医学院基础医学部针刺原理研究室神经生理、神经形态实验室; 上海第一医学院基础医学部针刺原理研究室神经生理、神经形态实验室;

【摘要】 作者试用卵自素-生物素-过氧化物酶复合物法(ABC法),观察大鼠端脑尾壳核、隔核及视前区亮氨酸脑啡肽的定位。结果看到脑啡肽样免疫反应存在于以上脑区,反应产物较为均匀地分布于神经细胞的胞浆及突起中,有些细胞的核区也有分布。在隔核部位,除外侧隔核外,内侧隔核也有明确的阳性反应。本文还结合针刺镇痛机理的研究进行了讨论。

【Abstract】 Using the avidin-biotin-complex (ABO), localizations of leucineenkephalin in the caudateputamen, the septal nuclei and the preoptic area of the rat telenceph alon were investigated. Enkephalin-like immunoreaotivity (ELI) was present in the perikarya and neuronal processes in the above brain areas. The reaction product in immunoreaetive neurons was more or less uniformly distributed in the cytoplasm and in some oases, in the nucleus. In the septal area, ELI containing perikarya was found not only in the lateral septal nucleus, but also in the medial septal nucleus. The results are discussed from the aspect of the mechanism of acupuncture analgesia.

  • 【文献出处】 上海第一医学院学报 , 编辑部邮箱 ,1985年04期
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