【摘要】 用人A型红细胞作为抗原免疫BALB/C小鼠,取其脾细胞与小鼠骨髓瘤细胞NS-1进行细胞杂交。从五批细胞融合中选取五个克隆细胞株,其中一株命名C₁₁细胞株,经七次克隆化培养,体外传代培养达八个多月,生长良好,继续分泌单克隆抗体。其单克隆抗体与不同血型红细胞（凝集法）和正常人外周血T、B淋巴细胞以及三个正常人B淋巴母细胞系补体依赖微量细胞毒方法作交叉试验,结果C₁₁细胞株产生的单克隆抗体只对A型红细胞发生反应与淋巴细胞抗原亦无交叉反应。更多还原

【Abstract】 The spleen cells from BALB/C mice immunized with human group-A red cells were fused with NS-1 myeloma cells. One monoclonal antibody derived from hybridoma cells, designated as C-11, was found to react with all human group-A red cells but not with red cells of group-B or group-O by hemagglutination assay. Using the technique of trypan-blue exclusion microlymphocytotoxicity, we found that this monoclonal antibody did not react against human peripheral T-cells, B-cells and three B lymphoblastoid cell lines established earlier by the authors. Our results demonstrate that the monoclonal antibody derived from C-11 is specific for human blood group-A red cells. The C-11 strain has been propagated stably in vitro for more than eight months without losing its ability to secrete antibody.更多还原