【摘要】 本文报道了三线镰刀菌M-20（Fusarium tricinctum）接种于已灭菌的玉米粒培养基中,于13℃培养30天后,提取培养物所得粗毒素。经二次硅胶柱层析纯化后,经丙酮-正己烷结晶,可获得白色针状结晶。根据它的熔点（151—2℃）R_f0.56,LD₅₀2.5,mg/kg。分子量466,FD质谱测定,¹H核磁共振谱及EI质谱,确证结晶为T-2毒素。更多还原

【Abstract】 Fusarium tricinctum M-20 was grown on corn medium at 13℃ for 30 days. Crude toxins were extracted from the culture. White crystalline toxin was obtained from crude toxins by Sillica gel column chromatograpy and crystallization from acetone-n-hexane. According to its m.p. （151—2℃）, E₁ （0.56, TLC 5% CH₃OH in CHCl₃）, ¹HNMR, LD₅₀ （2.59mg/kg）, MW （466, FD-MS）, fragment peaks （EI-MS）, the crystalline toxin was ascertained as T-2 toxin, 4, 15-diacetoxy 8-（isovaleyloxy）-12, 13-epoxy-trichothecene-9-ene-3-ol.更多还原